Techniques in Neuroscience

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Use information provided in the module plus your own research to answer ONE of the questions below.
The word limit for your essay is 1500 words + 10%, that is, 1650 words. The penalty for exceeding this limit is a deduction of 5 marks, and words beyond this will not be considered.
The word count includes everything in the main body of the text – headings, tables plus table
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1. Dr Bradshaw’s research group wants to study whether protein P is expressed in proliferating cells of the dentate gyrus. At present no antibody for protein P is available. Use your knowledge of immunohistochemistry gained from the module content AND your independent study to discuss experiments that will allow the researchers to answer the questions below. Provide ONE experiment for each question.
(a) How could the researchers Identify the cell-type(s) within the dentate gyrus that express protein P?
(b) How could the researchers confirm that the protein P expressing cells are adult neural stem cells?
(c) How could protein P expression be used to evaluate the effects of diet OR exercise on adult hippocampal neurogenesis in the mouse?
2. Consider a situation where human induced pluripotential cell (iPSC) lines have been generated from individuals with a neuropsychiatric disorder of your choice (for example, schizophrenia). Explain and critically evaluate the potential benefits of using human iPSCs over other methodologies for studying this disorder. What type of neural
cell would you differentiate the iPSCs into in order to better understand this neuropsychiatric disorder and why?
3. Many major breakthroughs in our understanding and treatment of disease have been possible because of studies using animal models. Debate the advantages and limitations of using animal models to understand diseases of the human brain. Discuss in your answer two examples where such studies have proved beneficial and at least one example where they have failed to deliver the desired outcomes. For more information on Techniques in Neuroscience see this:

Techniques in Neuroscience

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