Pharma Research


Ragnarsson L, et al. Extracellular surface residues of the alpha1B-adrenoceptor critical for G protein-coupled receptor function. Molecular Pharmacology 87: 121-129, 2015.

Don't use plagiarized sources. Get Your Custom Essay on
Pharma Research
Just from $13/Page
Order Essay


REMINDER:  If you are using any direct quotes from the paper (or other sources) in your answer, please remember to use quotation marks and/or sources.



  1. (2) Read the abstract for the paper.

What is the hypothesis they propose to test?




  1. (4) Using the abstract AND the rest of the paper:
  2. How do they propose to test this hypothesis?




  1. What region of the receptor do they plan to examine?




  1. Once they create the mutations in the receptor, how do they plan to assess the function of the mutant receptors (HINT: what properties of receptor function do they plan to assess?)




  1. (2) The researchers employed a technique referred to as “alanine scanning mutagenesis”. MANY scientific papers would have included literature citations in the References section that would explain the method and the rationale for its use. This paper, however, does not.


List two approaches that you could use for an internet search strategy to find out about the technique of “alanine scanning mutagenesis”. Include your rationale for each approach

  1. Approach: One approach I could take is to type in exactly what I am looking for in Google Scholar, and clink on a link and filter the results.






  1. Approach: Another approach is going to a database website, and performing an advanced search.



Rationale: If I went on the to American Society of Microbiology, it would allow me to perform an advance search, that would allow the for the filtering of key phrases,



  1. (4) Using one of your search strategies, identify another scientific paper published within the past two years that has also utilized this strategy:


  1. List the citation here:



  1. Using the resources you have through the online Scott Library, you want to find the fastest way to obtain the article (so that you could read it). Describe how and where you would obtain the article.



  1. (4) Give a brief description of “alanine scanning mutagenesis”. Why is the rationale for using the amino acid alanine (it may be helpful to show amino acid structures).






  1. (4) Back to the paper: Table 1 summarizes the data from 5 different sets of experiments.
  2. Which set(s) of data showed the MOST residues with statistically significant differences from the WT receptor? ______________



  1. Which set(s) of data showed the FEWEST residues with statistically significant differences from the WT receptor? ________________



  1. Which residue is the most “interesting” based on the data presented in this table? ______



  1. Now look at Figure 8 to determine the location of this residue in the sequence (TMH2, ECL1, etc). Where is this residue located? ________________


The take home message from looking at the question from multiple perspectives and from multiple sets of experimental data is that you cannot just look at one property in order to get the big picture! If the authors had ONLY looked at the EC50 values for a receptor agonist (here they used Norepinephrine), they would have reached very different and perhaps erroneous conclusions! (Remember that EC 50 is a measure of potency).

Grab 16% Discount On This Paper
Pages (550 words)
Approximate price: -

Why Choose Us

Quality Papers

ACME Homework provides the best top-grade academic writing services in compliance with our customers’ instructions. Have your paper written by a certified professional online college homework help writer to produce only high-quality essays with zero plagiarism.

Professional Academic Writers

You can now choose from a pool of online college homework help writers. Choose your writer and have them write the best content for you. ACME Homework has, over the years, secured a team of the most reliable, experienced, and qualified writers. You can, therefore, trust that your assignment is in good hands.

Affordable Prices

We know that students have very limited budgets. And for that, we always strive to provide only the best, most affordable online college homework help services to our customers. Our goal is to provide top-quality assignment help services to all customers at the lowest, most affordable prices.

On-Time delivery

At, we pay strict attention to deadlines. We recommend you to check out clients’ reviews for assurance that we will complete your assignments within the set deadlines. You can, therefore, trust that your paper will be done within and before the set deadline. Until now, we have not missed a single deadline.

100% Originality

Our homework helper experts write only 100% original and plagiarism-free content for all of our clients. We also have a Quality Assurance Department team that goes through all work submitted by our writers multiple times. You can, therefore, rest assured that any signs of plagiarized or unoriginal content will be rejected before it reaches your portal.

Customer Support 24/7 expert writers are always available 24/7 for customers who need assistance with using our website. You don’t have to check your watch the next time you want to have your assignment written. Our customer support is always available round the clock and ready to listen to your queries. Feel free to contact us via the Chat window or support email:

Try it now!

Grab 16% Discount On This Paper

We'll send you the first draft for approval by at
Total price:

How it works?

Follow these simple steps to get your paper done

Place your order

Fill in the order form and provide all details of your assignment.

Proceed with the payment

Choose the payment system that suits you most.

Receive the final file

Once your paper is ready, we will email it to you.

Our Services

For years now, has stood as a leader in providing its customers with the best online college homework help service in the industry. And all you have to do is provide us with the details of your order. Leave everything else to us. We’ve always got you covered.


Essay Writing Services

Since we launched, deserved the best online “college homework help status” thanks to our essay ordering, writing, and delivery process. We deliver nothing but excellence in our results. Our essay writing services include impeccable grammar, zero-plagiarism, proper structure, and conformance to guidelines.


Admission and Business Papers

Our top-quality online college homework help services guarantee that you will be accepted into your desired university. You just need to fill out your admission and business papers, and our team of online homework help workers will handle the rest. We will help you achieve and secure the best positions in your admissions forms.


Editing and Proofreading

At, we have a skilled writing and editing team that’s dedicated to creating, editing, and restructuring for all types of papers. Our online college homework help editing and proofreading team will check, paraphrase, and correct any grammar mistakes on your paper before submitting the final document to you.


Technical Papers

At, we pride ourselves in having writers in almost all fields, even the most technical ones. You never have to worry about your paper being too technical for our certified online college homework help writers to handle. ACME Homework’s team of writers can handle even the most complex writers. We will match your paper to the most competent writer that we believe will handle your paper the best way possible.

                                                                                     Place Order