Focus your attention only on the role of the cytoskeleton in the three
main parts of the SARS-CoV-2 virus (covid19) life cycle: viral entry (internalization),
transport via early endosomes to the ER-Golgi for replication, synthesis and packaging,
and lastly, transport via late endosomes back to the plasma membrane for exocytosis of
new viruses. For each of these phases, explain which cytoskeletal components are
most likely involved, that is, choose microtubules or microfilaments as the major
polymer (or “railroad tracks”) the virus uses, and which motor protein (dynein, kinesin
and myosin) would be involved. You need to describe specific experiments you will use
to confirm your hypothesis. In the experiments you design, be sure to indicate which
drug(s) you will use, how you will fluorescently label the molecule you want to follow,
and the kind of microscopy (confocal epifluorescence or electron microscopy) you will
use. You should also describe how you will measure replication of the virus, for example
“Real Time PCR.”
Just need it added to the document I already have written.
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